{"id":47886,"date":"2022-04-02T07:27:22","date_gmt":"2022-04-02T07:27:22","guid":{"rendered":"https:\/\/assignmenttutors.net\/blog\/osmosis-week-3-experiment-answer-sheet\/"},"modified":"2022-04-02T07:27:22","modified_gmt":"2022-04-02T07:27:22","slug":"osmosis-week-3-experiment-answer-sheet","status":"publish","type":"post","link":"https:\/\/assignmenttutors.net\/blog\/osmosis-week-3-experiment-answer-sheet\/","title":{"rendered":"Osmosis week 3 experiment answer sheet"},"content":{"rendered":"
\n

Please submit to the Week 3 Experiment dropbox no later than Sunday midnight.<\/span><\/em><\/p>\n

SUMMARY OF ACTIVITIES FOR WEEK 1 EXPERIMENT ASSIGNMENT<\/span><\/p>\n

\u00a0<\/span><\/p>\n

\u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/span>Experiment 3 Exercise 1<\/span>\u00a0\u2013 Diffusion: Movement of Solutes across a Membrane<\/span><\/p>\n

\u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/span>Experiment 3<\/span>\u00a0Exercise 2\u00a0\u2013 Osmosis: Movement of Water across a Membrane<\/span><\/p>\n

\u00a0<\/span><\/p>\n

Experiment 3 Exercise 1: Diffusion – Movement of Solutes across a Membrane<\/span><\/p>\n

\u00a0We will be using\u00a0dialysis tubing\u00a0to simulate a semipermeable membrane. This tubing allows small molecules (e.g., water, ions, glucose) to pass while preventing large molecules (e.g., macromolecules like proteins, starch, glycogen) from moving across. \u00a0Be sure you have read over the suggested material before starting this exercise and that you have reviewed the following animations:<\/span><\/p>\n

Experimental Design<\/span><\/p>\n

\u00a0<\/span><\/p>\n

A.\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0The\u00a0dialysis bag\u00a0we will use is permeable to water and small molecules (e.g., less than 500 g\/mol) and impermeable to large molecules (e.g., more than 500 g\/mol).<\/span><\/p>\n

B.\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0The dialysis bag is filled with a mixture of\u00a0glucose\u00a0(molecular weight = 180 g\/mol) and\u00a0protein\u00a0(molecular weight = 10,000 g\/mol) dissolved in water. A small\u00a0subsample\u00a0of the dialysis bag contents is saved and will be used in Step 4.<\/span><\/p>\n

C.\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0The dialysis bag is then placed into a beaker of\u00a0water. A small\u00a0subsample\u00a0of beaker water is also saved and is to be used in Step 4 as well.<\/span><\/p>\n

The presence or absence of glucose and protein will be determined using\u00a0indicators. Indicators change colors in the presence certain materials. The two tests that we\u2019ll use are the\u00a0Benedict\u2019s test\u00a0for simple sugars (e.g., glucose) and theBiuret test\u00a0for the presence of proteins.<\/span><\/p>\n

\u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/span>If glucose is present, the Benedict\u2019s indicator will turn\u00a0green. If no glucose is present, the solution will be\u00a0blue.<\/span><\/p>\n

\u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/span>If protein is present, the Biuret indicator will turn\u00a0violet. \u00a0If the solution remains\u00a0clear, then no protein is present.<\/span><\/p>\n

4.\u00a0\u00a0\u00a0 <\/span><\/span>The subsample of dialysis bag solution and the beaker water are tested for the presence of glucose and protein. SeeTable 1\u00a0below for the results.<\/span><\/p>\n

5.\u00a0\u00a0\u00a0 <\/span><\/span>The dialysis bag is then left in the beaker of water for 60 minutes.<\/span><\/p>\n

6.\u00a0\u00a0\u00a0 <\/span><\/span>At the end of 60 minutes, the dialysis bag solution and the beaker water are again tested for the presence of glucose and protein. See\u00a0Table 1\u00a0below for the results.<\/span><\/p>\n

\u00a0<\/span><\/p>\n

Table 1<\/span>. Results of testing of the dialysis bag and beaker contents at the beginning and end of the Experiment.<\/span><\/p>\n

\u00a0<\/span><\/p>\n

Questions<\/span><\/p>\n

1.\u00a0\u00a0\u00a0 <\/span><\/span>Summarize the results regarding the presence (+) or absence (-) of glucose and protein in the dialysis bag and beaker in Table 2 below (4 pts):<\/span><\/p>\n

\u00a0<\/span><\/p>\n

\u00a0\u00a0<\/span><\/p>\n

2.\u00a0\u00a0\u00a0 <\/span><\/span>Explain the movement or lack of movement of protein and glucose across the dialysis bag membrane (4 pts)<\/span><\/p>\n

3.\u00a0\u00a0\u00a0 <\/span><\/span>Which solution, that in the bag or that in the beaker, is\u00a0hypotonic\u00a0compared with the\u00a0protein\u00a0solution (2 pts)?<\/span><\/p>\n

4.\u00a0\u00a0\u00a0 <\/span><\/span>What factors affect the movement of molecules across a semipermeable membrane? Which factor plays the greatest role in biological systems (4 pts)?<\/span><\/p>\n

\u00a0<\/span><\/p>\n

5.\u00a0\u00a0\u00a0 <\/span><\/span>Briefly explain what active transport is and how it differs from passive transport, especially in terms of concentration gradients (4 pts).<\/span><\/p>\n

\u00a0<\/span><\/p>\n

\u00a0<\/span><\/p>\n

\u00a0<\/span><\/p>\n

Experiment 3 Exercise 2: Osmosis – The Movement of Water across a Membrane<\/span><\/p>\n

\u00a0<\/span><\/p>\n

Before starting, let\u2019s see what you know about the terms\u00a0hypotonic,\u00a0isotonic\u00a0and\u00a0hypertonic. Examine the diagrams below. Note that the small green circles represent dissolved\u00a0solutes\u00a0like salt, glucose, and amino acids. You can assume that the additional space surrounding the solutes is\u00a0water\u00a0and that the tan area is INSIDE the cell.<\/span><\/p>\n

\u00a0<\/span><\/p>\n

Question<\/span><\/p>\n

\u00a0<\/span><\/p>\n

1.\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0Define\u00a0each term below in terms of solute concentration outside compared to the inside of the cell. You do\u00a0not\u00a0need to explain which direction water will move\u00a0(3 pts).<\/span><\/p>\n

a.\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0Hypotonic –<\/span><\/p>\n

\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 b.\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0Isotonic –\u00a0
\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0 c.\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0Hypertonic –<\/span><\/p>\n

\u00a0<\/span><\/p>\n

Procedure<\/span><\/p>\n

1.\u00a0\u00a0\u00a0 <\/span><\/span>Open the following website to get started:<\/span><\/p>\n

The Biology Place. No Date. Osmosis: Movement of Water across Membranes
<\/span>http:\/\/www.phschool.com\/science\/biology_place\/biocoach\/biomembrane1\/osmosis.html<\/span><\/a><\/p>\n

B.\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0Read over the information presented and then\u00a0Click\u00a0on\u00a0\"\"<\/span><\/p>\n

C.\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0Then,\u00a0Click\u00a0on\u00a0.\u00a0 Read through the information presented and be sure to click on\u00a0Animate\u00a0beneath the illustration.<\/span><\/p>\n

2.\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0What concentration of salt is isotonic to animal cells\u00a0(1 pts)?\u00a0<\/span><\/p>\n

\u00a0<\/span><\/p>\n

3.\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0When cells are in isotonic solution, is there movement of water into or out of the cell?\u00a0 If so, describe this movement\u00a0(3 pts). \u00a0<\/span><\/p>\n

\u00a0<\/span><\/p>\n

    \n
  1. Click<\/span>\u00a0on\u00a0\u00a0.\n

    E.\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0Read through the information presented and be sure to click on\u00a0Animate\u00a0beneath the illustration. When ready, answer the following question.<\/p>\n

    <\/span><\/li>\n<\/ol>\n

    \u00a0<\/span><\/p>\n

    \u00a0<\/span><\/p>\n

    4.\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0Describe the net movement of water molecules when cells are placed in a\u00a0hypotonic\u00a0solution.\u00a0 Explain\u00a0why\u00a0water moves this way\u00a0(3 pts).<\/span><\/p>\n

    \u00a0Procedure\u00a0(continued)<\/span><\/p>\n

    G.\u00a0\u00a0\u00a0\u00a0\u00a0Read through the information presented and be sure to click on\u00a0Animate\u00a0beneath each of the illustrations. Answer the following questions. Your answers should incorporate the\u00a0terminology\u00a0used in the animations.<\/span><\/p>\n

    5.\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0What happens to an animal cell when placed in a\u00a0hypotonic\u00a0solution\u00a0(2 pts)?<\/span><\/p>\n

    \u00a0<\/span><\/p>\n

    \u00a0<\/span><\/p>\n

    6.\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0What happens to plant cells when placed in a\u00a0hypotonic\u00a0solution? What accounts for the difference in outcomes between animal cells and plant cells\u00a0(3 pts)?<\/span><\/p>\n

    \u00a0<\/span><\/p>\n

    \u00a0<\/span><\/p>\n

    Procedure<\/span>\u00a0(continued)<\/span><\/p>\n

    8.\u00a0\u00a0\u00a0 <\/span><\/span>Click<\/span>\u00a0on\u00a0\u00a0\"\"<\/span><\/p>\n

    I.\u00a0\u00a0\u00a0\u00a0\u00a0Then,\u00a0 Read through the information presented and be sure to click on\u00a0Animate\u00a0beneath the illustration. Answer the following question.<\/span><\/p>\n

    \u00a0<\/span><\/p>\n

    7.\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0Describe the net movement of water molecules when cells are placed in a\u00a0hypertonic\u00a0solution.\u00a0 Explain\u00a0why\u00a0water moves this way\u00a0(3 pts).<\/span><\/p>\n

    \u00a0<\/span><\/p>\n

    Procedure<\/span>\u00a0(continued)<\/span><\/p>\n

    10.\u00a0 <\/span><\/span>Click<\/span>\u00a0on\u00a0\u00a0<\/span><\/p>\n

    K.\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0Read through the information presented and be sure to click on\u00a0Animate\u00a0beneath the illustration. Answer the following questions.<\/span><\/p>\n

    8.\u00a0\u00a0\u00a0\u00a0Compare and contrast what happens to\u00a0plant\u00a0and\u00a0animal\u00a0cells when placed in a hypertonic solution. Be sure to use proper terminology\u00a0(4 pts).<\/span><\/p>\n

    9.\u00a0\u00a0\u00a0\u00a0Based on what you learned in this exercise, explain why\u00a0salt\u00a0might make a good weed killer\u00a0(3 pts).<\/span><\/p>\n

    \u00a0<\/span><\/p>\n

    Week 3 Experiment Grading Rubric<\/span><\/p>\n

    \u00a0<\/p>\n

    \u00a0<\/span><\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

    Please submit to the Week 3 Experiment dropbox no later than Sunday midnight. SUMMARY OF ACTIVITIES FOR WEEK 1 EXPERIMENT ASSIGNMENT \u00a0 \u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Experiment 3 Exercise 1\u00a0\u2013 Diffusion: Movement of Solutes across a Membrane \u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Experiment 3\u00a0Exercise 2\u00a0\u2013 Osmosis: Movement of Water across a Membrane \u00a0 Experiment 3 Exercise 1: Diffusion – Movement of Solutes […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[16],"tags":[],"class_list":["post-47886","post","type-post","status-publish","format-standard","hentry","category-education"],"_links":{"self":[{"href":"https:\/\/assignmenttutors.net\/blog\/wp-json\/wp\/v2\/posts\/47886","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/assignmenttutors.net\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/assignmenttutors.net\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/assignmenttutors.net\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/assignmenttutors.net\/blog\/wp-json\/wp\/v2\/comments?post=47886"}],"version-history":[{"count":0,"href":"https:\/\/assignmenttutors.net\/blog\/wp-json\/wp\/v2\/posts\/47886\/revisions"}],"wp:attachment":[{"href":"https:\/\/assignmenttutors.net\/blog\/wp-json\/wp\/v2\/media?parent=47886"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/assignmenttutors.net\/blog\/wp-json\/wp\/v2\/categories?post=47886"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/assignmenttutors.net\/blog\/wp-json\/wp\/v2\/tags?post=47886"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}