{"id":23063,"date":"2022-01-05T13:08:00","date_gmt":"2022-01-05T13:08:00","guid":{"rendered":"https:\/\/assignmenttutors.net\/blog\/enzymes-temperature-ph-and-specificity-hands-on-labs-inc\/"},"modified":"2022-01-05T13:08:00","modified_gmt":"2022-01-05T13:08:00","slug":"enzymes-temperature-ph-and-specificity-hands-on-labs-inc","status":"publish","type":"post","link":"https:\/\/assignmenttutors.net\/blog\/enzymes-temperature-ph-and-specificity-hands-on-labs-inc\/","title":{"rendered":"Enzymes: temperature, ph, and specificity hands-on labs, inc."},"content":{"rendered":"<div>\n<p>0Enzymes: Temperature, pH, and Specificity<br \/>Hands-On Labs, Inc.\u00a0 Version 42-0054-00-01<br \/>Lab Report Assistant\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment\u2019s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students\u2019 writing of lab reports by providing this information in an editable file which can be sent to an instructor.<br \/>Exercise 1: Enzyme Specificity<br \/>Observations\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <br \/>Data Table 1: Glucose Concentration<br \/>Wells\u00a0Concentration of Glucose<br \/>a\u00a0<br \/>b\u00a0<br \/>c\u00a0<br \/>d\u00a0<br \/>e\u00a0<br \/>\u00a0<br \/>Questions \u00a0<\/p>\n<p>A.\u00a0 What determines a person\u2019s ability to digest lactose?<br \/>B.\u00a0\u00a0 Which of the wells showed a positive result for glucose? Explain the results.<br \/>C.\u00a0\u00a0 Explain why testing for glucose is used to determine the activity of the enzyme lactase.<br \/>D.\u00a0 Explain the experimental conditions for the five different wells.<br \/>\u00a0<br \/>Data Table 2: Presence of glucose in wells indicating lactase activity at various temperatures<br \/>Well\u00a0Time (min)\u00a0Concentration of Glucose<\/p>\n<p>a\u00a05\u00a0<br \/>\u00a010\u00a0<br \/>\u00a015\u00a0<br \/>\u00a020\u00a0<br \/>b\u00a05\u00a0<br \/>\u00a010\u00a0<br \/>\u00a015\u00a0<br \/>\u00a020\u00a0<br \/>c\u00a05\u00a0<br \/>\u00a010\u00a0<br \/>\u00a015\u00a0<br \/>\u00a020\u00a0<br \/>Exercise 2: Enzymes and Temperature<br \/>Observations \u00a0<br \/>\u00a0<br \/>Questions: \u00a0<br \/>A.\u00a0 Graph the effect of temperature on the activity of the enzyme lactase.<br \/>B.\u00a0\u00a0 What happens when an enzyme is boiled? Is this effect reversible?<br \/>C.\u00a0\u00a0 Based on your experiment results, what is the optimal temperature for lactase function?<br \/>D.\u00a0 Explain what happens as far as the effectiveness of the enzyme at the freezing temperature. Can this effect be overcome when the temperature rises?<br \/>\u00a0<br \/>Exercise 3: Enzymes and pH<br \/>Observations \u00a0<\/p>\n<p>Data Table 3: Glucose in wells a-d indicates enzyme activity at various pH levels<br \/>Well\u00a0pH\u00a0Concentration of Glucose<br \/>a\u00a03.5\u00a0<br \/>b\u00a05.0\u00a0<br \/>c\u00a06.8\u00a0<br \/>d\u00a011.0\u00a0<\/p>\n<p>Questions \u00a0<br \/>A.\u00a0 Graph the data placing glucose concentrations on the y-axis and the pH values on the x-axis.<br \/>B.\u00a0\u00a0 What was the effect of pH on the enzyme lactase? Is this true for all enzymes?<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>0Enzymes: Temperature, pH, and SpecificityHands-On Labs, Inc.\u00a0 Version 42-0054-00-01Lab Report Assistant\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment\u2019s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[16],"tags":[],"class_list":["post-23063","post","type-post","status-publish","format-standard","hentry","category-education"],"_links":{"self":[{"href":"https:\/\/assignmenttutors.net\/blog\/wp-json\/wp\/v2\/posts\/23063","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/assignmenttutors.net\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/assignmenttutors.net\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/assignmenttutors.net\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/assignmenttutors.net\/blog\/wp-json\/wp\/v2\/comments?post=23063"}],"version-history":[{"count":0,"href":"https:\/\/assignmenttutors.net\/blog\/wp-json\/wp\/v2\/posts\/23063\/revisions"}],"wp:attachment":[{"href":"https:\/\/assignmenttutors.net\/blog\/wp-json\/wp\/v2\/media?parent=23063"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/assignmenttutors.net\/blog\/wp-json\/wp\/v2\/categories?post=23063"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/assignmenttutors.net\/blog\/wp-json\/wp\/v2\/tags?post=23063"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}